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Course Contents

1. Solvent Extraction: Basic principle of solvent extraction, The Distribution Coefficient, The Distribution Ratio, Solvent Extraction of Metals, Analytical Separations, Multiple Batch Extractions, Counter current Distribution, Solid-Phase Extraction, Solvent Extraction by Flow Injection Method. 2. Electrophoresis: Introduction, History, Principle, Theory, Instrumentation, Working of Electrophoresis Gel and its types, Application of Electrophoresis. Analytical Chemistry II (Practicals) Flame Emission / Spectrophotometry: • Determination of λmax of KMnO4 and K2Cr2O7 by using spectrophotometer. • Determination of Sodium in tap water by using Flame Photometer. • Determination of Potassium in tap water by using Flame Photometer. • Find out the calcium in chalk sample by flame photometry. • Determination of Ba by flame photometry. • Verification of Beer and Lambert Law. • Ultraviolet spectrophotometric determination of Aspirin, Phenacetin and Caffeine in APC samples.

Course Synopsis

During this course the principle and major application of solvent extraction will be covered. It will also include the various ways of doing extraction. The course will also cover another separation technique, Electrophoresis, that is extremely important in Bioanalytical chemistry. The course will cover its theory, Principle, Instrumentation, working and applications. In lab course applications of two important analytical techniques will covered i.e. Spectrophotometry and Flame photometry also their use in qualitative and quantitative analysis on lab scale.

Course Learning Outcomes

Students are able to learn two important separation techniques in Analytical chemistry: Solvent Extraction and Electrophoresis. Student are able to learn about their advantages as well as limitations. They will understand the theory, instrumentation and working of the techniques with total lab protocols regarding these techniques. Students will improve their lab skills as well as instrumentation skills during the lab work. They will be able to handle the important basic analytical instruments; spectrophotometer and Flame photometer. They will also be able to apply these instruments in qualitative and quantitative analysis.

Solvent extraction of Metals

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Gel Electrophoresis and its Applications

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Principle of Electrophoresis

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Solvent Extraction

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Solid Phase Extraction

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Book Title : Modern Analytical Chemistry - Chapter 12
Author : David T. Harvey
Edition : 1st
Publisher : McGraw Hill USA

Book Title : Fundamentals of Analytical Chemistry- Chapter 30
Author : Skoog, West, Holler, Crouch
Edition : 8
Publisher : Thomson Books USA

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