Dr. Rasheeda Bashir

Tenured Associate Professor

Department of Biotechnology

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Profile
Research Publications

Experience

Date	Title	Institute
January 2014-to date	Assistant Professor (TTS)	Department of Biotechnology, Lahore College for Women University, Lahore
August 2012 - August 2013	Assistant Professor (IPFP)	Department of Biotechnology, Lahore College for Women University, Lahore
June 2009- July 2010	Teacher Assistant	Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore

Honor and Awards

Ph.D Scholarship, University of the Punjab, Lahore, Pakistan (2005-2006)

Receive best performance award in the field of education by frog-e- taleem gold Medal award 2013

Memberships

Pakistan Zoological Society

Graduate Students /Postdocs/Undergraduate
/Students/Honour Students

Ph.D Thesis Supervision

On Going

Name of Student: Maria Rafique

Topic: Production and immunological evaluation of polyvalent vaccine of avian influenza (AIV-H9) and fowl cholera (Pasterulla multocida)

Name of Student: Khazeema Yousaf
Topic: Genetic causes of Glaucoma in Pakistani population

MS Students Supervised
Year	Name	Title
2019	Sadaf Bashir	Association of MCP-1 rs1024611 polymorphism with type II diabetic foot ulcer patients in Lahore, Punjab
2019	Umera Kauser	The association of VEGF rs699947 polymorphism in type II diabetic patients with or without diabetic foot ulcer from Punjab
2019	Iman Fatima	Screening of genetic variants p.M98K of optineurin gene in primary open angle glaucoma patients from Punjab
2019	Zernish Shabbir	Genetic variants analysis of p.D658G OF WDR36 gene in patients affected with primary open angle glaucoma from
2018	Hira Ali	Frequency of STAT4 gene single nucleotide polymorphism rs7574865 contribution to systematic lupus erthmatous in Pakistani population
2018	Samina Maqbool	Association of superoxide dismutase2 gene polymorphism rs4880 and open angle glaucoma in Pakistani population
2018	Ramish Shafique	Frequency of D835 mutation in FLT3 gene of leukemia of Pakistani population
2018	Resham Saleem	Genetic polymorphism and PACG: The association of SOD2 SNP rs4880 with PACG in Pakistan
2018	Aimen Naveed	Analysis of DeltaF508 mutation of CFTR gene in chronic pancreatitis patients of Pakistani population.
2018	Sana Aziz	Evaluation of antimicrobial activity of silver nanoparticles against wound infections
2017	Bushra Irfan	Association of Hepatocytes Growth factors gene polymorphism with primary angle closure glaucoma in Pakistani population
2017	Mehak Khalid	Genetic analysis of P-Type Adenosine Triphosphate Gene (ATP7B) in Pakistani Patients Affected with Wilson’s Disease
2017	Zunaira Tariq	The frequency of cationic trypsinogen (PRSS1) and serine protease inhibitor, kazal type 1 (SPINK1) gene mutations in Pakistani patients affected with chronic pancreatitis
2016	Harem Ali	Frequency of the p.G61E mutation in recessively inherited primary open glaucoma in Pakistani population.
2016	Rooha Tariq	Association of TP53 polymorphisms (rs1042522) and (rs17878362) with recessive primary open angle glaucoma in Pakistani population
2015	Rabia Mushtaq	Correlation between genotype and phenotype in adult primary open angle glaucoma in Pakistan with mutations in exon 3 of the MYOC gene
2015	Neelam Zaib	Antimicrobial susceptibility profiling and characterization of CTX-M gene in ESBL producing Klebsiella Pneumonia
2015	Sana Irum	Role of Myocillin (MYOC) gene sequence variation in juvenile open angel glaucoma (JOAG) in Pakistani population: Correlation between genotype and phenotype
2014	Khazeema Yousaf	Contribution of CYP1B1 gene mutations associated with primary congenital glaucoma in Pakistani population
2014	Hafsa Tahir	Screening of cytochrome p450 mutations associated with primary open angle glaucoma in Pakistani population
2013	Marukh Sanai	Genetic analysis of recessive glaucoma in Pakistan
BS/M.sc. Students Supervised
2019	Tayyaba Mahmood	In vitro mutagenicity assay of the cooking oils before and after and after heat treatment
2019	Resham razzaq	Assessment of mutagenic effects of artificial sweeteners by microsomal assay
2019	Nadia Tahir	Comparative mutagenic assessment of cooking oil and ghee before and after heat treatment by Salmonella typhimurium assay system
2019	Samra	Evaluation of mutagenicity of artificial sweeteners by Salmonella typhimurium assay
2018	Razia Gahni	Gamma sterilization of surgical gauzes using cobalt-60 for elimination of residential microbial flora
2018	Noreen Mustafa	Use of gamma radiation to eliminate bioburden from syringes
2018	Iqra Shafique	A systematic study for the optimization and comparison of gamma irradiation doses with ethylene oxide sterilization on surgical gauzes
2018	Ammara Tahir	Dose dependent effect of gamma radiation on the selected eye ointments
2018	Amber Iftikhar	Effect of gamma radiation sterilization on eye ointment to eliminate the bioburden
2017	Afsheen Nasir	Isolation and identification of (Gram positive) from burn wounds and its antibiotic sensitivity
2017	Sana Saleem	Microbial flora (Gram negative) of burn wounds and its antibiotic sensitivity
2017	Ameera Saleem	Effect of different physico-chemical factors on yogurt quality and production
2017	Andleeb Afzal	Effect of mix and pure culture of (lactic acid producing bacteria) on quality of local yogurt
2017	Nida Akram	Isolation and identification of lactic acid producing bacteria from different brands of yogurt
2016	Anum Asif	Effect of gamma radiation on fungal flora of rice (Basmati and Sella)
2016	Imrana Akram	Optimization of gamma dose to irradiate micro flora and insects from rice .To maximize rice safety and storability
2016	Rida Sadaqat	Dose optimization and assessment of proximate for the evaluation of nutrients and antioxidants of export quality irradiated basmati rice
2016	Fatima Anwar	Enhancement of shelf life and nutrient evaluation of locally grown export quality irradiated Sella rice
2015	Braira Wahid	Reducing the Risk of Nosocomial Illnesses by sterilization of Nasal Oxygen Cannula Using Gamma Radiation (Cobalt – 60 Source)
2015	Resham Saleem	An Assessment of Microbial Load on Nelaton Tube and its Effective Sterilization by gamma radiation
2015	Bakht Afroze	Targeting the Infection Causing Microbes on Tracheostomy Tube by the Optimizing the Dose of Gamma Radiation (CO-60).
2015	Hafiza Fizzah Zulfiqar	Estimation of microbial load and dose optimization for gamma irradiation of endotracheal tube
2015	Aneeqa javed	Targeting the viable microorganisms on oxygen mask through optimized gamma radiation dose
2014	Maryam Qayyum	Reduction of microbial load of surgical forceps to minimal extent by gamma radiation
2014	Roha Tariq	Irradiation of cannula by cobalt – 60 source (gamma radiation) to ensure its safe usage by eliminating microbial load
2014	Tahira Haider	Targeting the residing infection causing microbes using optimal dose of gamma radiation on feeding tubes (nasogastric tube)
2014	Faryal Akhtar	Optimization of gamma radiation dose to minimize the microbial load on surgical scissors
2014	Mazia Amber	Identification and elimination of microbial load over commercially available medical drip set

Service Activity

Advisory and Administrative Services

Member Doctoral committee

Member research ethical review committee

Member Purchase committee (Chemicals)

Coordinator BS Program

BS Admission committee member

Member of synopsis defense committee (BS, MS, PhD.)

Brief Statement of Research Interest

Medical Genetics
Medical Microbiology
Immunology

Impact Factor Journals

Rasheeda Bashir, Khazeema Yousaf, Hafsa Tahir, Marukh Sanai, Seema Qayyum, Shagufta Naz, Sadaf Naz. 2018. Clinical variability of CYP1B1 gene variants in Pakistani primary congenital glaucoma families. Pakistan journal of Medical Association. 68( 8) 1205-1211 (ISSN:0030-9982; Impact factor 0.616)
Imtiaz A,Belyantseva IA, Beirl AJ, Fenollar-Ferrer C, Bashir R, Bukhari I, Bouzid A, Shaukat U, Azaiez H, Booth KT, Kahrizi K, Najmabadi H, Maqsood A Wilson EA, Fitzgerald TS, Tlili A, Olszewski R, Lund M, Chaudhry T, Rehman AU, Starost MF, Waryah AM, Hoa M, Dong L, Morell RJ, Smith RJH, Riazuddin S,Masmoudi S,Kindt KS, Naz S, Friedman TB.2018. CDC14A phosphatase is essential for hearing and male fertility in mouse and human. Human Molecular Genetics. 27 (5) 780-798 (ISSN: 0964-6906; Impact factor 5.340)
Naz S, Imtiaz A, Mujtaba G, Maqsood A, Bashir R, Bukhari I, Khan MR, Ramzan M, Fatima A, Rehman AU, Iqbal M, Chaudhry T, Lund M, Brewer CC, Morell RJ, Friedman TB.2017. Genetic causes of moderate to severe hearing loss point to modifiers. Clinical Genetics. 91: 589-598 (ISSN: 0009-9163. Impact factor 3.326)

Komal Najam, Imran Altaf, M Ashraf, M Adil Rasheed, Faiza Saleem, Neelma Munir, Rasheeda Bashir. 2017. In vitro evaluation of mutagenicity and genotoxicity of sitagliptin alone and in combination with artificial sweeteners. Tropical Journal of Pharmaceutical Research. 16 (8) 1841-1847 (ISSN: 1596-5996; Impact factor 0.569)

Bashir R, Afroze B, Zulfiqar HF, Saleem R, Altaf I, Saleem F, Aslam F, Naz S. 2016. Microbiological Load of Ethylene Oxide Sterilized Medical Devices and its Elimination by Cobalt 60 Source. Journal of College for Physicians and Surgeon Pakistan. 26(6) 486-489 (ISSN: 1022-386X; Impact factor 0.554)

Rizwana Raheel, Muhammad Ashraf, Aqeel Javeed, Rasheeda Bashir, Neelma Munir and Imran Altaf. Evaluation of antiviral and cytotoxic potential of ethanolic extract of Acacia nilotica against peste des petits ruminants virus in vitro cell culture. Indian Journal of Animal Sciences. 86 (11) 1274–1275 (ISSN 0367-8318; Impact Factor: 0.22)

Ishrat Imran, Imran Altaf, Muhammad Ashraf, Aqeel Javeed, Neelma Munir, Rasheeda Bashir. 2016. In vitro evaluation of antiviral activity of leaf extracts of Azadirachta indica, Moringa oleifera, and Morus alba against the foot and mouth disease virus on BHK-21 cell line. Science Asia. 42: 392-396. (ISSN: 15131874; Impact factor 0.343)

Rasheeda Bashir, Hafsa Tahir, Khazeema Yousaf Shagufta Naz , Sadaf Naz. 2015. Homozygous p.G61E mutation in a consanguineous Pakistani family with co-existence of juvenile-onset open angle glaucoma and primary congenital glaucoma. Gene. 570: 295–298 (ISSN: 0378-1119; Impact factor 2.415)
Midhat Salman, Rasheeda Bashir, Ayesha Imtiaz, Azra Maqsood, Ghulam Mujtaba, Muddassar Iqbal & Sadaf Naz. Mutations of GJB2 encoding connexin 26 contribute to non-syndromic moderate and severe hearing loss in Pakistan. European Archive of Otorhinolaryngology 272 (8) 2071-2075 (ISSN: 0937-4477; Impact factor 1.660)
Faiza Saleem, Aisha Younas, Rasheeda Bashir, Shagufta Naz, Neelma Munir and Abdul Rauf Shakoori. 2014. Molecular Cloning and Characterization of Exochitinase A Gene of Indigenous Bacillus thuringiensis Pakistan Journal of Zoology, 46(6): 1491-1501. (ISSN: 0030-9923; Impact Factor: 0.400)
Neelma Munir, Ayesha Saleha Iqbal, Imran Altaf, Rasheeda Bashir, Nadia Sharif , Faiza Saleem and Shagufta Naz. 2014. Evaluation of antioxidant and antimicrobial potential of two endangered plant species Atropa belladonna and Matricaria chamomilla. African Journal of traditional, complementary and alternative medicine. 11(5) 111-117 (ISSN 0189-6016; Impact factor 0.56)

Rasheeda Bashir, Mahrukh Sanai, Adnan Azeem, Imran Altaf, Faiza Saleem , Sadaf Naz. 2014. Contribution of GLC3A locus to Primary Congenital Glaucoma in Pakistani population. Pakistan Journal of Medical Science. 30(6): 1341-1345 (ISSN: 1682-024X; Impact factor 0.166)

Bashir R, Imtiaz Z A, Fatima A, Alam A, Naz S. 2013.The association of the c.42_52del11 mutation in TPRN with progressive or stable hearing loss. Biochemical Genetics. 51(5-6):350-357. (ISSN: 0006-2928; Impact factor 1.02)

Khan R, Bashir R, Naz S. 2013. Mutations in SLC26A4 cause moderate to severe hearing loss in Pakistani families. Biochemical Genetics. 51: 514–523. (ISSN: 0006-2928; Impact factor 1.02)

Rasheeda Bashir, Amara Fatima, Sadaf Naz. 2011. Prioritized sequencing of the second exon of MYO15A reveals a new mutation segregating in a Pakistani family with moderate to severe hearing loss. European Journal of Medical Genetics. 55(2) 99-102 (ISSN, 1769-7212; Impact factor 2.178)

Rasheeda Bashir, Amara Fatima, Sadaf Naz. 2010. Mutations in CLDN14 are associated with different hearing threshold. The Journal of Human Genetics. 55(11) 767-670.(ISSN: 0340-6717 impact factor 2.570)
R Bashir, A Fatima, S Naz. 2010. A deletion mutation in SANS results in atypical Usher syndrome. Clinical Genetics78(6)601-603.( ISSN‎: ‎0009-9163; Impact factor 3.128)

International publications in ISI Journals

1.Neelma Munir, Samra Pervaiz, Rukhama Haq, Rasheeda Bashir and Shagufta Naz. 2018. Effect of gamma radiation on post-harvest spoilage of spinach. International Journal Of Biology Pharmacy And Allied Sciences, 7(6) 991-998 (ISSN: 2277–4998; ISI Indexed)

2.Rasheeda Bashir, Neelam Zaib, Imran Altaf, Faiza Saleem, Qamar Sultana, and Shagufta Naz. 2016. Optimization of PCR for rapid detection of CTX-M gene in ESBL producing Klebsiella pneumoniae clinical isolates from Punjab, Pakistan. Malaysian journal of Microbiology, 12 (5) 365-369 (ISSN : 1823-8262; ISI indexed)

3.Imran Altaf, Anika Khalid , Jawad Nazir , Waseem Shahzad ,Rasheeda Bashir. 2016. Outer membrane proteins and lipopolysaccharides mediated antibody response against bovine Pasteurella multocida type B-2. Malaysian journal of Microbiology. 12 (5): 370-375. (ISSN : 1823-8262; ISI indexed)

4.Neelma Munir, ayesha Saleha Iqbal, Imran Altaf, Rasheeda Bashir And Shagufta Naz.2016. Phytochemical Analysis Of Extracts Of Atropa Belladonna And Matricaria Chamomila Obtained By Microwave Extraction Method. International Journal of Biology, Pharmacy and Allied Science. 5(1): 144-152. (ISSN: 2277–4998; ISI Indexed)

5.Abdul Muqeet Khan , Imran Altaf , Shaguftasaeed , Mateen Abbas, , Asma Waris , Rukhama Haq , Rasheeda Bashir. 2016. Mycotoxin (Aflatoxin & Ochratoxin) And Pesticide Residues In Capsicum Frutescents In Punjab, Pakistan. International Journal of Biology, Pharmacy and Allied Sciences. 5(7): 1646-1654. (ISSN: 2277–4998;ISI Indexed)

6.Rasheeda Bashir, Braira Wahid, Aneeqa Javed, Imran Altaf, Neelma Munir, Rukhama Haq, Shagufta Naz. 2015. Reducing the risk of nosocomial illnesses by targeting the viable microflora on oxygen mask and nasal oxygen cannula through COBALT-60 SOURCE. International Journal of Biology, Pharmacy and Allied Sciences. 4(10): 6098-6106. ( ISSN: 2277–4998; ISI Indexed)

Books /Manuals Published

A practical Manual of “Basic Technique in Molecular Biology “(2015) by Ch. Publisher Lahore, Pakistan.
A Handbook of Microbiology” General Characteristic and Diagnosis of Pathologically Important Gram Positive Bacteria” (2015) by Khalid Publisher (Pvt) Limited, Urdu Bazaar, Lahore Pakistan.
A Practical manual of “Basic of Cell Culture Techniques” (2014) by Awami Publishers (PVT) LTD Lahore Pakistan.

Proceeding Published

Shagufta Naz, Rasheeda Bashir (2014) published proceeding on HEC funded one day workshop on “Emerging issues of cancers in Pakistan” Published by Ch. Publisher Anarkali Lahore, Pakistan.
Naz A. Imtiaz, R. Bashir, G. Mubtaja, A. Maqsood, I. Bukhari, A. Rehman, R. J. Morell, T. B. Friedman. (2014). Alleles of the reported deafness genes are major contributors to the etiology of moderate to severe hearing loss in Pakistani population. Publication date, 2014/10/21. Conference, American Society of Human Genetics, Issue, 2946T

Research Grants and Contracts

Research Grant as PI on HEC project No. NRPU/R&D/HEC/2738 entitled “Genetic Analysis of Disease Causing Genes for Glaucoma in Pakistani population” awarded by HEC; Award amount: 1.846 million. (2017-on going)
Research Grant as PI of 0.5 Million from HEC (2013) Research project titled “Genetic Analysis of Glaucoma in Pakistan”. Awarded by HEC. Completed.

Other Research or Creative Accomplishments

Patents Filed

“A process of Inoculum preparation to enhance the composting efficiency” submitted by Dr. Neelma Munir, Ms. Aisha Amin, Dr. Imran Altaf, Dr. Rasheeda Bashir, and Prof. Dr. Shagufta Naz in Gazette No. 192/2016

“A process for identification and isolation of fourteen novel strains of Klebsiella pneumoniae subsp pneumonia with CTX-M gene from Pakistani population” by Dr. Rasheeda Bashir, Ms. Neelam Zeb, Prof. Dr. Shagufta Naz, Dr. Imran Altaf, Dr. Faiza Saleem, Dr. Neelma Munir, Dr. Farheen Aslam, Ms Rukhama Haq in Gazette No. 220/2015

“Identification and isolation of two genetically heterogeneous novel Pakistani strains of klebsiella pneumonia subsp oxitoca BT2 and BT8 with CTX-M gene from Pakistani pneumonia patients" submitted by Dr. Rasheeda Bashir, Ms. Neelam Zeb, Prof. Dr. Shagufta Naz, Dr. Imran Altaf, Dr. Faiza Saleem, Dr. Neelma Munir, Dr. Farheen Aslam, Ms Rukhama Haq Published in Gazette No. 221/2015

“Identification of two novel Pakistani strains Acinetobacter baumanii PK35 and Acinetobacter baumanii PK39 with new Delhi-Metallo –B- Lactamase-1 (BLANDM-1) gene isolated from Pakistani children’’ submitted by Dr. Farheen Aslam, Hira Lodi, Khwaja Ahmad Irfan Waheed, Prof. Dr. Shagufta Dr. Rasheeda Bashir. Published in Gazette No. 203/2015

“Identification of three novel Pakistani strains Klebsiella pneumoniae PK1, Klebsiella pneumoniae PK9, with new Delhi-Metallo B- Lactamase-1 (BLANDM-1) gene isolated from Pakistani children, submitted by Dr. Farheen Aslam, Hira Lodi, Khwaja Ahmad Irfan Waheed, Prof. Dr. Shagufta Dr. Rasheeda Bashir. Published in Gazette No. 204/2015

Selected Professional Presentations

Oral Presentations

New trends in natural sciences II: public health ,food nutrition & safety24^th -26^th Oct, 2018 organized by Department of Biotechnology And Department of Zoology LCWU, Lahore, Pakistan

1^st International Conference on Biotechnology Challenges and Opportunities in Pakistan. 11^th -12^th October, 2017 organized by Lahore Garrison University

International Conference on “New Trends in Natural Sciences: from Basics to Applied. 25^th -27^th October, 2017 organized by Department of Biotechnology, And Department of Zoology LCWU, Lahore, Pakistan

3^rd International Conference on Biotechnology: Challenges and Opportunities in Pakistan. 8^th -9^th Feb, 2017 University of South Asia, Lahore, Pakistan

Research Colloquium. 16^th -19^th Jan, 2017 DFDI, LCWU, Lahore, Pakistan

3^rd Regional Conference on Biosensors, Biodegradation, Biochips and Biotechnology, 20^th -22th April 2016 AIMST University Malaysia

13^th Medical Microbiology & Infectious Diseases Conference 25^th -26^th March 2016 organized by Society of Pakistan Infectious Diseases Lahore, Pakistan

2^nd International Conference on Biotechnology : Challenges and Opportunities in Pakistan 24^th -25^th February , 2016 organized by University of South Asia, Lahore, Pakistan

HEC funded one day workshop on “Emerging issues of cancers in Pakistan May 2014HEC Funded / Department of Biotechnology, LCWU, Lahore, Pakistan

Poster Presentations:

2^nd International Conference on Biotechnology: Challenges and Opportunities in Pakistan. 24th-25th Feb, 2016 University of South Asia, Lahore, Pakistan

3rd International Conference on Biotechnology: Challenges and Opportunities in Pakistan. 8^th -9^th Feb, 2017 organized by University of South Asia, Lahore, Pakistan

Department of Biotechnology

Biotechnology